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Literature summary extracted from

  • Haass, W.; Stehle, M.; Nittka, S.; Giehl, M.; Schrotz-King, P.; Fabarius, A.; Hofmann, W.K.; Seifarth, W.
    The proteolytic activity of separase in BCR-ABL-positive cells is increased by imatinib (2012), PLoS ONE, 7, e42863.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.49 cyclinB1
-
 Homo sapiens
3.4.22.49 imatinib activation of separase proteolytic activity occurs exclusively in BCR-ABL-positive cells during imatinib treatment (0.00025-0.01 mM for 24 h) Homo sapiens
3.4.22.49 PP2A
-
 Homo sapiens
3.4.22.49 securing
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.49 centrosome
-
 Homo sapiens 5813
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.49 cohesin + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.49 Homo sapiens Q14674
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.49 phosphoprotein the enzyme is phosphorylated at serine residue 1126 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.49 fibroblast
-
 Homo sapiens
-
3.4.22.49 HL-60 cell
-
 Homo sapiens
-
3.4.22.49 K-562 cell
-
 Homo sapiens
-
3.4.22.49 LAMA-84 cell
-
 Homo sapiens
-
3.4.22.49 U-937 cell
-
 Homo sapiens
-
3.4.22.49 urothelial cell
-
 Homo sapiens
-
3.4.22.49 UROtsa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.49 cohesin + H2O
-
 Homo sapiens ?
-
 ?

Expression

EC Number Organism Comment Expression
3.4.22.49 Homo sapiens decreased separase protein levels are observed in BCR-ABL-positive and -negative cells treated with imatinib in a dose dependent manner down

General Information

EC Number General Information Comment Organism
3.4.22.49 physiological function separase is required for the separation of sister-chromatides in mitotic anaphase, triggers centriole disengagement during centrosome duplication. In cancer, separase is frequently overexpressed, pointing to a functional role as an aneuploidy promoter associated with centrosomal amplification and genomic instability Homo sapiens